HER Imaging and Molecular Interaction Mapping in Breast Cancer
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Partner: Mitochondrial Dynamics Group, Max Planck Institute for Biophysical Chemistry

Project Leader
Stefan Jakobs

Group members
Peter Ilgen (Postdoc)
Rita Schmitz-Salue (Technician)



References
Grotjohann, T., Testa, I., Leutenegger, M., Bock, H., Urban, N.T., Lavoie-Cardinal, F., Willig, K.I., Eggeling, C., Jakobs, S.*, and Hell, S.W.* (*shared corr. author), (2011). Diffraction-unlimited all-optical imaging and writing with a photochromic GFP. Nature, doi: 10.1038/nature (Pubmed)

Brakemann, T., Stiel, A.C., Weber, G., Andresen, M., Testa, I., Grotjohann, T., Leutenegger, M., Plessmann, U., Urlaub, H., Eggeling, C., Wahl, M.C., Hell, S.W. *, and Jakobs, S. (2011). A reversibly photoswitchable GFP-like protein with fluorescence excitation decoupled from switching. Nature Biotechnology, doi: 10.1038/nbt (Pubmed)

Wurm, C.A., Neumann, D., Lauterbach, M.A., Harke, B., Egner, A., Hell, S.W., and Jakobs, S. (2011). Nanoscale distribution of mitochondrial import receptor Tom20 is adjusted to cellular conditions and exhibits an inner-cellular gradient. Proc Natl Acad Sci USA, 108, 13546-51 (Pubmed)

Kukat, C., Wurm, C.A., Spåhr, H., Falkenberg, M., Larsson, N., and Jakobs, S. (2011). Super-resolution microscopy reveals that mammalian mitochondrial nucleoids have a uniform size and frequently contain a single copy of mtDNA. Proc Natl Acad Sci USA, 108, 13534-9 (Pubmed)

Andresen, M., Stiel, A.C., Fölling, J., Wenzel, D., Schönle, A., Egner, A., Eggeling, C., Hell, S.W., and Jakobs, S. (2008). Photoswitchable fluorescent proteins enable monochromatic multilabel imaging and dual color fluorescence nanoscopy. Nature Biotechnology, 26, 1035-1040


Nanoscopy with DARPins

In classical immunochemistry, antibodies of the IgG type are used to label cellular structures. Due to the size of the primary and secondary antibody (about 10 nm each) the size of the observed structure increases by 30-40 nm. The size of the DARPins (3 nm) may open up a resolution regime that is not addressable with conventional labeling techniques. We will set up, optimize and perform STED nanoscopy with sub-50 nm resolution on various cultured breast cancer cell line models as well as on tissue samples using DARPins and antibodies to evaluate the nanoscopic distribution of HER2 and related proteins.

See also: Mitochondrial Dynamics Group, Max Planck Institute homepage.


Funding bodies

Max-Planck-Society


DFG Research Center Molecular Physiology of the Brain




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