HER Imaging and Molecular Interaction Mapping in Breast Cancer
FP7 imagint
 
  Partner: King's College London
  Richard Dimbleby Laboratory of
  Cancer Research in ImagInt
Project Leader
Prof. Tony Ng

Group members
Dr. Gregory Weitsman
Dr. Gargi Patel

Introduction

The group is among one of the few in the world which brings together research scientists from different disciplines including advanced optical physics, molecular and cell biology and tumour pathology and clinical cancer treatment; working collaboratively to develop a variety of new technologies in the field of cancer cell and tissue imaging, which researchers hope will help evaluate patients’ disease progression.


Role in the “Imagint Consortium”

Development of FRET-FLIM based assays for protein-protein interactions and their application for imaging and evaluation of cancer status and aggressiveness in human samples.

Figure. Automated microscopy of human cancer tissue arrange in tumour microarray format (A) allow detailed imaging of tumor morphology and application of antibodies based FRET/FLIM assay for protein-protein interaction (B) allow to find tumours with high (1) or low (2) interaction between ErbB2 and ErbB3 proteins.


Selected Publications

1. Ng T, Squire A, Hansra G, Bornancin F, Prevostel C, Hanby A, Harris W, Barnes D, Schmidt S, Mellor H, Bastiaens PI, Parker PJ. Imaging protein kinase Calpha activation in cells. Science. 1999 Mar 26;283(5410):2085-9. PMID: 10092232

2. An unbiased screen identifies DEP-1 tumor suppressor as a phosphatase controlling EGFR endocytosis. Tarcic G, Boguslavsky SK, Wakim J, Kiuchi T, Liu A, Reinitz F, Nathanson D, Takahashi T, Mischel PS, Ng T, Yarden Y. Curr Biol. 2009 Nov 17;19(21):1788-98. PMID: 19836242

3. Peter M, Ameer-Beg SM, Hughes MK, Keppler MD, Prag S, Marsh M, Vojnovic B, Ng T. Multiphoton-FLIM quantification of the EGFP-mRFP1 FRET pair for localization of membrane receptor-kinase interactions. Biophys J. 2005 Feb;88(2):1224-37. 2004 PMID: 15531633

4. Kelleher MT, Fruhwirth G, Patel G, Ofo E, Festy F, Barber PR, Ameer-Beg SM, Vojnovic B, Gillett C, Coolen A, Kéri G, Ellis PA, Ng T. The potential of optical proteomic technologies to individualize prognosis and guide rational treatment for cancer patients. Target Oncol. 2009 Sep;4(3):235-52. 2009 PMID: 19756916

5. Fruhwirth GO, Fernandes LP, Weitsman G, Patel G, Kelleher M, Lawler K, Brock A, Poland SP, Matthews DR, Kéri G, Barber PR, Vojnovic B, Ameer-Beg SM, Coolen AC, Fraternali F, Ng T. How Förster resonance energy transfer imaging improves the understanding of protein interaction networks in cancer biology. Chemphyschem. 2011;12(3):442-61. PMID: 1328516
 

Funding Bodies










 


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